Optopub: 16th February 2023
Location: Solna, Stockholm
Sponsors: Hübner Photonics - Cobolt
An Optopub on High Performance Lasers Enabling Innovative Life Science Applications, sponsored and hosted by Hübner Photonics/Cobolt in Solna.
The evening included scientific talks, drinks, food and an exclusive soft opening of HÜBNER Photonics new office expansion.
Real-time single-molecule 3D tracking in E. coli
Presented by Elias Amselem, Researcher at Dpt. of Cell and Molecular Biology
We have little detailed knowledge about the intracellular dynamics of fundamental processes such as gene regulation or protein translation; the intracellular environment is very different from the dilute conditions where most of what we know about biochemistry has been learned. Here, we present a 3D single-molecule tracking system based on cross¼entropy. Putting the system into practice, we track single fluorophores within the Escherichia coli bacterium.A fluorescent marker is attached to the E. coli trigger factor (TF), a bacterial chaperone protein that binds to translating ribosomes close to the peptide exit tunnel. The system can locate a reporter in a volume of size 0.95×0.95×1.40 of 0.84 ms at a photon count rate of 120kHz.
Transient state (TRAST) spectroscopy and imaging of cellular and molecular states and conditions – exploiting
the sensing side of fluorophore blinking kinetics
Presented by Jerker Widengren, Exp Biomol. Physics group, Dept. Applied Physics, Royal Institute of Technology (KTH)
Dark state transitions of fluorophores are central for all forms of fluorescence-based, single-molecule andsuper-resolution microscopy and spectroscopy, mainly as limiting factors in single molecule studies, at the same time prerequisites for super-resolution imaging techniques. This overview will highlight an additional aspect of such transitions, namely that they can be used to sense a manifold of biomolecular environments, dynamics and interactions. Principles for how they can be imaged will be described, as well as application examples in cells
and tissues.
The B-BRIGHTER project
Presented by Dr. Gustaf Mårtensson, Expert Complex Fluids at Mycronic Adjunct researcher at CBH-KTH
The B-BRIGHTER project is an EU-financed research project with the goal of developing a novel, light-based
bioprinting technology. The project utilises continuous-wave lasers, acousto-optics, galvanomirrors and custom
hydrogel-based bioinks to make this concept a reality.
HÜBNER Photonics and Cobolt
Presented by Håkan Karlsson, CEO of Cobolt and Head of HÜBNER Photonics
